The P value or calculated probability is the probability of finding the observed, or more extreme, results when the null hypothesis is true – the definition of ‘extreme’ depends on how the hypothesis is being tested. P is also described in terms of rejecting the null hypothesis (H 0 ) of a study question when H 0 is actually true, however, it is not a direct probability of this state.
The null hypothesis is usually an hypothesis of "no difference" e.g. no difference between blood pressures in group A and group B. Define a null hypothesis for each study question clearly before the start of your study.
The only situation in which you should use a one sided P value is when a large change in an unexpected direction would have absolutely no relevance to your study. This situation is unusual; if you are in any doubt then use a two sided P value.
The term significance level (alpha) is used to refer to a pre-chosen probability and the term "P value" is used to indicate a probability that you calculate after a given study.
The alternative hypothesis (H 1 ) is the opposite of the null hypothesis; in plain language terms this is usually the hypothesis you set out to investigate. For example, question is "is there a significant (not due to chance) difference in blood pressures between groups A and B if we give group A the test drug and group B a sugar pill?" and alternative hypothesis is " there is a difference in blood pressures between groups A and B if we give group A the test drug and group B a sugar pill".
If your P value is less than the chosen significance level then you reject the null hypothesis i.e. accept that your sample gives reasonable evidence to support the alternative hypothesis. It does NOT imply a "meaningful" or "important" difference; that is for you to decide when considering the real-world relevance of your result.
The choice of significance level at which you reject H 0 is arbitrary. Conventionally the 5% (less than 1 in 20 chance of being wrong), 1% and 0.1% (P < 0.05, 0.01 and 0.001) levels have been used. These numbers can give a false sense of security.
In the ideal world, we would be able to define a "perfectly" random sample, the most appropriate test and one definitive conclusion. We simply cannot. What we can do is try to optimise all stages of our research to minimise sources of uncertainty. When presenting P values some groups find it helpful to use the asterisk rating system as well as quoting the P value:
P < 0.05 *
P < 0.01 **
P < 0.001
Most authors refer to statistically significant as P < 0.05 and statistically highly significant as P < 0.001 (less than one in a thousand chance of being wrong).
The asterisk system avoids the woolly term "significant". Please note, however, that many statisticians do not like the asterisk rating system when it is used without showing P values. As a rule of thumb, if you can quote an exact P value then do. You might also want to refer to a quoted exact P value as an asterisk in text narrative or tables of contrasts elsewhere in a report.
At this point, a word about error. Type I error is the false rejection of the null hypothesis and type II error is the false acceptance of the null hypothesis. As an aid memoir: think that our cynical society rejects before it accepts.
The significance level (alpha) is the probability of type I error. The power of a test is one minus the probability of type II error (beta). Power should be maximised when selecting statistical methods. If you want to estimate sample sizes then you must understand all of the terms mentioned here.
The following table shows the relationship between power and error in hypothesis testing: